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. 2018 Sep 3;8:278. doi: 10.3389/fcimb.2018.00278

Figure 2.

Figure 2

Effects of over-expression of hsa-miR-494-3p on EV71 infection. RD and HEK 293 cells were transfected with hsa-miR-494-3p mimics or the negative control (NC) for 48 h, and then followed by EV71 infection for 2, 4, 6, 12 h at an MOI of 2. Virus titers in culture supernatants from RD (A) and HEK 293 (B) were measured at the indicated time post-infection. The cells were collected at different time points as indicated. RD (C) and HEK 293 (D) cell lysates were blotted with anti-EV71 VP1 and anti-β actin antibodies. β actin was used as an internal control. Next, qRT-PCR was employed to determine the EV71 VP1 mRNA levels in RD (E) and HEK 293 (F) cells. The data shown are the mean ± standard deviation. The experiments were repeated three times (†P < 0.05, *p < 0.01 by ANOVA).