Figure 6.

Cytokine production by HSPCs in response to C. albicans and the TLR2 agonist. (A) Schematic protocol (as described in section Materials and Methods) to obtain secretomes. WT Lin⁻ cells were cultured at a density of 50,000 cells in 250 μl of a serum-free medium containing FL and SCF, in the presence or absence of Pam₃CSK₄ or inactivated C. albicans yeasts for 3 days. Then, secretomes (conditioned media) were collected. (B) TNF-α and IL-6 levels in the secretomes were measured by ELISA. (C) CCL2, CCL3, and CCL9 levels were determined using a mouse cytokine array (as described in section Materials and Methods). Data are expressed as means ± SD of pooled data from two experiments. **P < 0.01, ***P < 0.001, and ****P < 0.0001 with respect to control secretome.