Fig. 5.

Coisolated strains are not compatible. CFU counts for each coincubation spot for pairwise coincubations with squid 1 (A) and squid 2 (B and C) isolates. Asterisks indicate P < 0.02 (Student’s t test) indicating a statistically significant decrease in a strain’s CFUs at 5 h compared with 0 h for each coincubation. A dash (“—”) indicates vasA_2 mutants. Fluorescence microscopy images were taken after 24 h. (Scale bars, 2 mm.) All experiments were performed at least three times and a representative experiment is shown (n = 4).