Fig. 3.

POGLUT2 and POGLUT3 are responsible for O-glucosylation of mouse NOTCH1 EGF11. (A) EICs of glycoforms of the EGF11 peptide from NOTCH1 EGF1-36-Myc-His6 produced in WT HEK293T cells (clone AB3), POGLUT2 KO cells (clone EB5), POGLUT3 KO cells (clone HF8), or POGLUT2 and POGLUT3 double-KO cells (clone TG2). Black line, unglucosylated peptide; blue line, O-glucosylated peptide. A single asterisk indicates β-hydroxylation of N in peptide. (B) Ratios of peak areas of β-hydroxylated versus non–β-hydroxylated peptides were quantified by determining the total peak area from each EIC. The data were from three independent transfection experiments. Error bars show SEM. *P < 0.05; **P < 0.01. n.s., not significant.