Fig. 1.

Efferocytosis of apoptotic E. coli-infected cells triggers PGE₂ production. BMDCs were previously treated or not with indomethacin (10 µM) and then cocultured in the presence of IACs, at the ratio 1:3 for 18 h. (A) Schematic figure illustrating experimental procedure. (B) Concentration of PGE₂ and cytokines were measured by ELISA in the supernatants derived from resting BMDC, BMDC cocultured with noninfected apoptotic cells (BMDC+AC), IACs only (IAC), BMDC cocultured with IAC (CM), or indomethacin-treated BMDC cocultured with IAC (CM/Indo). Data represent mean ± SEM of at least three independent experiments performed in triplicate. ND, not detected. *P < 0.05 compared with BMDC; ^#P < 0.05 compared with CM.