Fig. 6.

In vivo inhibition of PGE₂ synthesis or EP4 signaling improves Th17 cell population in the colonic tissue of C. rodentium-infected mice. Mice were orally infected or not with C. rodentium and treated every other day with indomethacin (5 mg/kg), daily with L-161,982 (EP4 antagonist) (10 mg/kg) or vehicle for 7 d. On the eighth day of infection, colons were harvested. (A) Scheme of treatment. (B) PGE₂ levels quantified by ELISA in the colonic tissue. (C) The percentage of TCRβ⁺CD4⁺IL17A⁺ T cells in the colon were assessed by flow cytometry and demonstrated by representative dot plots and bar graph. (D) Measurement of body weight throughout the experiment. (E) The colon length of different groups of animals was measured after killing. Data represent mean ± SEM of two independent experiments. n = 7–10. *P < 0.05 compared with control (vehicle); ^#P < 0.05 compared with infected (vehicle).