Fig. 7.

Indomethacin or EP4 antagonist treatment improves host defense against C. rodentium infection in mice. Mice were orally infected with C. rodentium and treated intraperitoneally every other day with indomethacin (5 mg/kg) or daily with L-161,982 (EP4 antagonist) (10 mg/kg) or vehicle for 7 d. On the eighth day of infection, colons were harvested. (A) The colonic tissue homogenates were plated for CFU counting per gram of tissue. (B) The expression of Il1r1, Il17a, Il22, and (C) antimicrobial peptides genes were analyzed by qPCR in the colonic tissue and represented as relative expression to Gapdh. (D) Representative H&E-staining sections of distal colonic tissues from untreated noninfected and vehicle, indomethacin, and EP4 antagonist-treated infected mice are shown. Areas of mononuclear cell infiltration and epithelial tissue injury are indicated by arrowheads. (Magnification: 100×.) Data represent mean ± SEM of two independent experiments. n = 7–10. *P < 0.05 compared with vehicle-treated infected mice group.