| DARPin off7 |
| Source |
Synthetic construct |
| DNA source |
Plasmid ID 1514_i, Department of Biochemistry, University of Zurich, Switzerland |
| Forward primer |
GGGAATTCCATATGAGAGGATCGCATCACCATCA (PE-2888) |
| Reverse primer |
CCGCTCGAGTTAATTAAGCTTTTGCAGGATTTCCGC (PE-2889) |
| Cloning vector |
pET-30a |
| Expression vector |
pRG2743 |
| Expression host |
E. coli BL21-CodonPlus (DE3)-RIL |
| Complete amino-acid sequence of the construct produced |
MRGSHHHHHHGSDLGRKLLEAARAGQDDEVRILMANGADVNAADNTGTTPLHLAAYSGHLEIVEVLLKHGADVDASDVFGYTPLHLAAYWGHLEIVEVLLKNGADVNAMDSDGMTPLHLAAKWGYLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN |
| DARPin 16 |
| Source |
Synthetic construct |
| DNA source |
Plasmid ID 496_i, Department of Biochemistry, University of Zurich, Switzerland |
| Forward primer |
GGGAATTCCATATGAGAGGATCGCATCACCATCA (PE-2888) |
| Reverse primer |
CCGCTCGAGTTAATTAAGCTTTTGCAGGATTTCCGC (PE-2889) |
| Cloning vector |
pET-30a |
| Expression vector |
pRG2744 |
| Expression host |
E. coli BL21-CodonPlus (DE3)-RIL |
| Complete amino-acid sequence of the construct produced |
MRGSHHHHHHGSDLGKKLLEAARAGQDDEVRILMANGADVNADDTEGNTPLHLVAVHGHLEIVEVLLKYGADVNAHDVWGQTPLHLAAYYDHLEIVEVLLKYGADVNADDDTGITPLHLAARWGHLEIVEVLLKYGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN |
