| Method |
Vapor diffusion, hanging drop |
Vapor diffusion, hanging drop |
Vapor diffusion, hanging drop |
| Plate type |
EasyXtal 15-well plate (Qiagen) |
EasyXtal 15-well plate (Qiagen) |
EasyXtal 15-well plate (Qiagen) |
| Temperature (K) |
292 |
292 |
292 |
| Protein concentration (mg ml−1) |
31 |
27 |
27 |
| Composition of reservoir solution |
0.15 M sodium chloride, 0.1 M sodium cacodylate pH 6.0, 2.0 M ammonium sulfate |
0.02 M
DL-glutamic acid, 0.02 M
DL-alanine, 0.02 M glycine, 0.02 M
DL-lysine, 0.02 M
DL-serine, 0.1 M Tris–Bicine pH 8.5, 25%(v/v) MPD, 25%(v/v) PEG 1000, 25%(v/v) PEG 3350 |
0.02 M
DL-glutamic acid, 0.02 M
DL-alanine, 0.02 M glycine, 0.02 M
DL-lysine, 0.02 M
DL-serine, 0.1 M Tris–Bicine pH 8.5, 25%(v/v) MPD, 25%(w/v) PEG 1000, 25%(w/v) PEG 3350 |
| Volume and ratio of drop |
3 µl, 1:2 mixture of protein and reservoir solutions |
3 µl, 1:1 mixture of protein and reservoir solutions |
3 µl, 1:1 mixture of protein and reservoir solutions |
| Volume of reservoir (ml) |
0.5 |
0.5 |
0.5 |
| Cryosolution |
20%(v/v) ethanol in mother liquor |
2%(w/v) xylitol in mother liquor |
Paraffin oil |
| Soaking time in cryosolution |
12 h |
16 h |
5 s |
