Table 2. Crystallization.

Construct	His6-TssA Nt1	TssA CTD	SeMet-TssA CTD
Method	Sitting-drop vapour diffusion	Sitting-drop vapour diffusion	Hanging-drop vapour diffusion
Composition of reservoir solution	0.16 M calcium acetate, 0.08 M sodium cacodylate buffer pH 6.5, 14.4%(w/v) PEG 8000, 20%(v/v) glycerol	0.1 M sodium chloride, 0.1 M Tris pH 8.0, 15%(v/v) ethanol, 5%(v/v) MPD	1 M ammonium sulfate, 2%(w/v) PEG 3350, 0.1 M bis-tris pH 6.0
Volume and ratio of drop	200 nl:200 nl	200 nl:200 nl	2 µl:1 µl (protein:reservoir)
Volume of reservoir (µl)	50	50	1000