Table 1.

The intra-day and inter-day precision and accuracy of celastrol in plasma samples.

	Intra-day			Inter-day
Spiked concentration (ng/mL)	Concentration measured (ng/mL)	Precision (%, RSD)	Accuracy (%, RE)	Concentration measured (ng/mL)	Precision (%, RSD)	Accuracy (%, RE)
0.2	0.18	6.28	−10.00	0.21	5.25	5.00
5	5.16	7.62	8.40	4.62	8.37	−7.60
75	79.54	8.31	6.05	71.05	5.10	3.19

Spiked concentration: concentrations of celastrol spiked in plasma samples; Concentration measured: concentration determined by LC-MS; Precision: relative standard deviation of three determinations; Accuracy: relative error of three determinations.