Table 2.

Stability of celastrol in plasma samples (n = 3).

Stability conditions	Spiked concentration (ng/mL)	Measured concentration (ng/mL)	Precision (RSD, %)	Accuracy (RE, %)
Short-term	0.2	0.18	5.62	−10.00
(room temperature	5	4.62	7.25	−7.60
for 12 h)	75	68.35	7.01	−8.87
Autosampler (24 h)	0.2	0.21	8.34	5.00
	5	5.37	5.76	7.40
	75	81.98	6.37	9.31
Three freeze/thaw	0.2	0.22	8.46	10.00
cycles (−40 °C)	5	5.43	7.34	8.60
	75	70.26	5.29	−6.32
Long-term (−40 °C	0.2	0.21	6.20	5.00
for 15 days)	5	4.51	8.61	−9.80
	75	69.35	7.29	−7.53