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Structure change of Ile50 and Leu54 in the M20-closed crystal at (a) 0.1, (b) 400, (c) 650 and (d) 800 MPa. In (c), the side chain of Ile50 is modeled with two conformations. The electron-density maps cover key structures and are shown at 1.0σ in (a) and (b) and 0.5σ in (c) and (d).
