Table 1.
Rf, Linear regression data for the calibration curve of rutin, quercetin, naringenin and gallic acid (n = 6).
| Parameters | Rutin | Quercetin | Naringenin | Gallic acid |
|---|---|---|---|---|
| Linearity range (ng/spot) | 200–1400 | 200–1400 | 100–1200 | 100–1200 |
| Regression equation | Y = 12.434x + 443.49 | Y = 10.08x + 216.85 | Y = 11.253x + 973.52 | Y = 11.082x + 446.41 |
| Correlation (r2) coefficient | 0.997 ± 0.0004 | 0.9982 ± 0.0001 | 0.9974 ± 0.0004 | 0.9981 ± 0.0001 |
| Slope ± SD | 12.434 ± 0.124 | 10.08 ± 0.029 | 11.253 ± 0.121 | 11.082 ± 0.041 |
| Intercept ± SD | 443.49 ± 11.547 | 216.85 ± 8.171 | 973.52 ± 12.301 | 446.41 ± 15.557 |
| Standard error of slope | 0.050 | 00.012 | 0.049 | 0.016 |
| Standard error of intercept | 4.713 | 3.335 | 5.021 | 6.349 |
| Rf | 0.52 ± 0.006 | 0.23 ± 0.005 | 0.56 ± 0.009 | 0.28 ± 0.006 |
| LOD | 33.03 ng band−1 | 9.67 ng band−1 | 35.574 ng band−1 | 12.32 ng band−1 |
| LOQ | 100.1 ng band−1 | 29.31ng band−1 | 107.8 ng band−1 | 37.35ng band−1 |