Table 6.

Robustness of the proposed RP-HPTLC method (n = 3).

	Rutin (300 ng/band)			Quercetin (300 ng/band)
Optimization condition	SD	%RSD	SEM	SD	%RSD	SEM
Mobile phase composition; (Acetonitrile: water)
(4:6)	2.154	0.543	0.879	3.641	0.914	1.486
(3.8:6.2)	2.235	0.562	0.912	3.459	0.869	1.411
(4.2:5.8)	1.914	0.484	0.781	3.215	0.810	1.312
Mobile phase volume (for saturation)
(18 mL)	2.112	0.533	0.862	3.925	0.981	1.602
(20 mL)	2.214	0.558	0.903	3.858	0.973	1.575
(22 mL)	2.175	0.548	0.889	3.815	0.960	1.557
Duration of saturation
(10 min)	2.231	0.563	0.911	3.835	0.967	1.565
(20 min)	2.262	0.571	0.923	3.792	0.954	1.547
(30 min)	2.218	0.561	0.905	3.866	0.966	1.578