Table 7.
Robustness of the proposed NP-HPTLC method (n = 3).
| Naringenin (300 ng/band) |
Gallic Acid (300 ng/band) |
|||||
|---|---|---|---|---|---|---|
| Optimization condition | SD | %RSD | SEM | SD | %RSD | SEM |
| Mobile phase composition; (Toluene: ethyl acetate: formic acid) | ||||||
| (6:4:0.8) | 4.151 | 1.394 | 1.695 | 3.133 | 1.044 | 1.278 |
| (5.8:4.2:0.8) | 3.963 | 1.338 | 1.617 | 3.395 | 1.129 | 1.385 |
| (6.2:3.8:0.8) | 4.514 | 1.509 | 1.842 | 3.744 | 1.249 | 1.528 |
| Mobile phase volume (for saturation) | ||||||
| (18 mL) | 4.243 | 1.424 | 1.732 | 3.417 | 1.139 | 1.394 |
| (20 mL) | 4.157 | 1.404 | 1.696 | 3.614 | 1.202 | 1.475 |
| (22 mL) | 4.323 | 1.445 | 1.764 | 3.442 | 1.149 | 1.405 |
| Duration of saturation | ||||||
| (10 min) | 4.212 | 1.414 | 1.719 | 3.442 | 1.147 | 1.405 |
| (20 min) | 4.146 | 1.401 | 1.692 | 3.413 | 1.135 | 1.393 |
| (30 min) | 4.293 | 1.435 | 1.752 | 3.435 | 1.146 | 1.403 |