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. 2018 Jun 28;56(1):309–317. doi: 10.1080/13880209.2018.1474932

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Detection of mucin secretion and membrane water channel expression in the transverse colon after QCT treatment. (A) Alteration of mucin level. Mucin secreted from crypt layer cells was stained with Alcian blue at pH 2.5, and images were observed at 400× magnification. Five to six rats per group were assayed in triplicate by Alcian blue staining. (B) The levels of MUC2 and AQP8 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of MUC2 and AQP8 mRNA were calculated based on the intensity of actin as an endogenous control. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means ± SD of three replicates. *p < 0.05 compared to the No treated group. #p< 0.05 compared to the Lop + Vehicle treated group.