Figure 2.

Effects of AEDKC on IgE-mediated passive cutaneous anaphylaxis. Mouse ear skin (n = 5/group) was sensitized with an intradermal injection of anti-DNP IgE (0.5 μg/site) for 48 h. AEDKC and Dexa were orally administered 2 h before the intravenous injection of DNP-HSA (1 mg/mouse) and 4% Evans blue (1:1) mixture. Thirty minutes later, the ears were collected to measure pigmentation. The dye was extracted as described in the Materials and methods section and detected using a spectrophotometer. (A) Representative photographic images of ears. (B) Graph data represent the mean ± SD (n = 5/group) of two independent experiments. *p < 0.05 compared with the DNP-HSA-challenged group. Dexa: dexamethasone.