Figure 4.

Compound 1 (1 μM) enhances expression of an oestrogen responsive reporter gene in transiently transfected MCF-7 cells (p < 0.05, n = 8). This effect was inhibited by the ER antagonist ICI 182,780 (1 μM) (p < 0.05), demonstrating that 1 acts through the ER. E2 was tested at (10 nM). Three independent experiments were performed, and all tests were performed in triplicate. Data were analysed using one-way ANOVA followed by Tukey’s multiple comparison test. DMSO vs. E2 p < 0.001; DMSO vs. 1p < 0.001; E2 vs. ICI + E2 p < 0.001; 1 vs. 1 +ICI p < 0.001.