Table 2.
Composition of EOs obtained from three populations of Thymus alternans growing in Slovakia (NS – village of Nová Sedlica, RUN – village of Runina, POL – village of Topoľa. For details of localities see part Plant material).
| N. | Componenta | RIb | RI LIT.c | Area%d |
IDe | ||
|---|---|---|---|---|---|---|---|
| NS | RUN | POL | |||||
| 1 | α-Thujene | 916 | 924 | 0.1 | trf | RI, MS | |
| 2 | α-Pinene | 921 | 932 | 0.3 | 0.1 | tr | Std |
| 3 | Camphene | 934 | 946 | 0.5 | 0.1 | 0.1 | Std |
| 4 | Sabinene | 959 | 969 | tr | tr | tr | RI, MS |
| 5 | β-Pinene | 962 | 974 | 0.3 | 0.2 | 0.1 | Std |
| 6 | 1-Octen-3-one | 968 | 972 | 0.2 | 0.1 | tr | RI, MS |
| 7 | 1-Octen-3-ol | 974 | 974 | 1.6 | 1.1 | 1.4 | Std |
| 8 | 3-Octanone | 979 | 979 | 0.2 | 0.1 | 0.1 | RI, MS |
| 9 | Myrcene | 981 | 988 | 4.0 | 1.9 | 1.3 | Std |
| 10 | 3-Octanol | 990 | 988 | 0.2 | 0.2 | 0.1 | RI, MS |
| 11 | Α-terpinene | 1009 | 1014 | 0.4 | 0.3 | 0.1 | RI, MS |
| 12 | p-Cymene | 1017 | 1020 | 0.3 | 0.3 | 0.1 | Std |
| 13 | Limonene | 1020 | 1024 | 0.4 | 0.3 | 0.2 | Std |
| 14 | 1,8-Cineole | 1021 | 1026 | 0.7 | 0.3 | 0.4 | Std |
| 15 | (Z)-β-Ocimene | 1032 | 1032 | 1.0 | 0.5 | 0.4 | RI, MS |
| 16 | (E)-β-Ocimene | 1041 | 1044 | 7.0 | 4.3 | 2.6 | RI, MS |
| 17 | γ-Terpinene | 1050 | 1054 | 1.0 | 1.0 | 0.2 | Std |
| 18 | cis-Sabinene hydrate | 1057 | 1065 | 2.4 | 2.3 | 0.7 | RI, MS |
| 19 | Terpinolene | 1079 | 1086 | 0.2 | 0.2 | 0.1 | Std |
| 20 | trans-Sabinene hydrate | 1089 | 1098 | 0.3 | 0.3 | 0.1 | RI, MS |
| 21 | Linalool | 1095 | 1095 | 2.7 | 1.7 | 6.4 | Std |
| 22 | n-Undecane | 1100 | 1100 | 0.2 | 0.1 | Std | |
| 23 | 1-Octen-3-yl acetate | 1110 | 1110 | 0.1 | 0.6 | 0.1 | RI, MS |
| 24 | cis-p-Menth-2-en-1-ol | 1113 | 1118 | tr | 0.2 | 0.1 | RI,MS |
| 25 | allo-Ocimene | 1124 | 1128 | 0.1 | tr | Std | |
| 26 | Camphor | 1132 | 1141 | 1.1 | 0.4 | 1.1 | Std |
| 27 | trans-Chrysanthemal | 1141 | 1153 | 0.2 | 0.3 | 0.2 | RI, MS |
| 28 | Nerol oxide | 1149 | 1154 | tr | 0.1 | 0.1 | RI, MS |
| 29 | Isoborneol | 1154 | 1155 | 1.1 | 0.5 | 1.0 | Std |
| 30 | Terpinen-4-ol | 1166 | 1174 | 1.8 | 1.8 | 1.0 | Std |
| 31 | Isogeranial | 1178 | 1174 | 0.4 | 0.4 | 0.4 | RI, MS |
| 32 | α-Terpineol | 1181 | 1186 | 0.5 | 0.3 | 0.5 | Std |
| 33 | Nerol | 1222 | 1227 | 1.9 | 4.9 | 3.1 | Std |
| 34 | Citronellol | 1225 | 1223 | 0.1 | 0.1 | 0.1 | Std |
| 35 | Thymol, methyl ether | 1229 | 1232 | tr | tr | tr | RI, MS |
| 36 | Neral | 1234 | 1235 | 5.1 | 5.4 | 4.9 | Std |
| 37 | Carvacrol, methyl ether | 1236 | 1241 | 0.1 | RI, MS | ||
| 38 | Geraniol | 1251 | 1249 | 3.3 | 6.0 | 6.2 | Std |
| 39 | Linalool acetate | 1254 | 1254 | 2.9 | tr | 2.4 | RI, MS |
| 40 | Geranial | 1265 | 1264 | 6.8 | 7.7 | 7.2 | Std |
| 41 | Isobornyl acetate | 1276 | 1283 | tr | tr | tr | Std |
| 42 | Carvacrol | 1299 | 1298 | 0.5 | Std | ||
| 43 | δ-Elemene | 1325 | 1335 | 0.1 | 0.1 | RI, MS | |
| 44 | Neryl acetate | 1360 | 1359 | 3.6 | 1.5 | 2.3 | Std |
| 45 | β-Bourbonene | 1369 | 1387 | 1.8 | 1.0 | 1.3 | RI, MS |
| 46 | Geranyl acetate | 1379 | 1379 | 0.3 | 0.9 | 1.1 | RI, MS |
| 47 | (E)-caryophyllene | 1402 | 1417 | 1.5 | 1.0 | 1.6 | Std |
| 48 | β-Copaene | 1413 | 1430 | 0.2 | 0.2 | 0.2 | RI, MS |
| 49 | α-Humulene | 1436 | 1454 | 0.2 | 0.4 | 0.2 | Std |
| 50 | allo-Aromadendrene | 1443 | 1458 | 0.7 | 0.1 | 0.1 | RI, MS |
| 51 | cis-Cadina-1(6),4-diene | 1449 | 1461 | 0.1 | RI, MS | ||
| 52 | (E)-β-Farnesene | 1450 | 1454 | 0.1 | RI, MS | ||
| 53 | Germacrene D | 1465 | 1484 | 6.7 | 7.4 | 7.4 | RI, MS |
| 54 | Bicyclogermacrene | 1480 | 1500 | 0.5 | 0.5 | 0.2 | RI, MS |
| 55 | α-Muurolene | 1487 | 1500 | 0.2 | tr | 0.1 | RI, MS |
| 56 | β-Bisabolene | 1498 | 1505 | 1.5 | 0.7 | 1.3 | RI, MS |
| 57 | (E,E)-α-Farnesene | 1501 | 1505 | 0.3 | 0.1 | 0.1 | RI, MS |
| 58 | δ-Cadinene | 1510 | 1522 | 1.1 | 0.2 | 0.4 | RI, MS |
| 59 | β-Sesquiphellandrene | 1512 | 1521 | 0.2 | 0.4 | tr | RI, MS |
| 60 | α-Cadinene | 1533 | 1537 | 0.1 | tr | RI, MS | |
| 61 | Hedycaryol | 1536 | 1546 | 2.9 | 3.6 | 1.4 | RI, MS |
| 62 | (E)-Nerolidol | 1559 | 1561 | 15.8 | 26.4 | 31.4 | Std |
| 63 | Germacrene D-4-ol | 1559 | 1574 | tr | tr | tr | RI, MS |
| 64 | Viridiflorol | 1584 | 1590 | 0.2 | Std | ||
| 65 | γ-Eudesmol | 1615 | 1630 | 0.4 | 0.1 | 0.2 | RI, MS |
| 66 | epi-α-Cadinol | 1626 | 1638 | 0.4 | 0.1 | 0.1 | RI, MS |
| 67 | epi-α-Muurolol | 1626 | 1640 | 0.4 | 0.3 | 0.2 | RI, MS |
| 68 | β-Eudesmol | 1631 | 1649 | 0.2 | 0.2 | 0.1 | RI, MS |
| 69 | α-Eudesmol | 1635 | 1652 | 0.3 | 0.3 | 0.2 | RI, MS |
| 70 | α-Cadinol | 1648 | 1652 | 1.8 | 0.8 | 0.6 | RI, MS |
| 71 | n-Nonacosane | 2900 | 2900 | 0.1 | 0.2 | 0.3 | Std |
| Total identified (%) | 91.0 | 91.4 | 93.2 | ||||
| Oil yield (%) | 0.2 | 0.2 | 0.1 | ||||
| Grouped compounds (%) | |||||||
| Monoterpene hydrocarbons | 15.6 | 9.2 | 5.1 | ||||
| Oxygenated monoterpenes | 35.3 | 34.6 | 39.1 | ||||
| Sesquiterpene hydrocarbons | 15.1 | 12.2 | 12.9 | ||||
| Oxygenated sesquiterpenes | 22.5 | 32.1 | 34.1 | ||||
| Others | 2.6 | 2.4 | 2.0 | ||||
a
Compounds are in order of elution from a HP-5MS column.
b
Retention index on HP-5MS column.
d
Percentage values are means of three determinations, with a RSD% for the main components below 10% in all cases.
e
Identification methods: MS, by comparison with the mass libraries Wiley, Adams and NIST 08; RI, by comparison with RIs-containing libraries (Adams, 2007; NIST 08, 2008; FFNSC 2, 2012); Std, by comparison with authentic standard.
f
tr, traces (mean value below 0.1%).