Table 3.

Robustness studies for lyoniside and lyoniresinol.

Change in mobile phase			Mean area ± SD		% RSD
Actual	Used	Level	LS	LR	LS	LR
4:3:0.4	3.5:3.5:0.4	−5	5995.9 ± 71.71	6339.46 ± 81.07	1.19	1.27
	4:3:0.4	0	6120.7 ± 62.07	6425.76 ± 60.96	1.01	0.94
	4.5:2.5:0.4	+5	6275.8 ± 78.45	6261.76 ± 72.53	1.25	1.15
Wavelength change
254	250	−4	6439.5 ± 60.71	6337.03 ± 68.46	0.94	1.08
	254	0	6357.8 ± 61.96	6432.36 ± 65.55	0.97	1.01
	258	+4	6441.8 ± 67.11	6268.03 ± 66.15	1.04	1.05