Table 1.
Validation data of developed method for catechin, diosgenin, gallic acid, quercetin, rutin and tannic acid.
| S. No. | Metabolites | Linearity range (μg/mL) | Regression equation | Coefficient ofcorrelation | LOD (μg/mL) | LOQ (μg/mL) |
|---|---|---|---|---|---|---|
| 1 | Catechin | 0.05–100 | Y = 30539.0X + 3962.7 | 0.98 | 0.08 | 0.24 |
| 2 | Diosgenin | 0.1–100 | Y = 2857.9X + 2213.6 | 0.98 | 0.17 | 0.51 |
| 3 | Gallic acid | 1–100 | Y = 330.8X − 2222.8 | 0.98 | 1.05 | 3.2 |
| 4 | Quercetin | 0.1–100 | Y = 2825.6X + 5561.1 | 0.99 | 0.38 | 1.15 |
| 5 | Rutin | 1–100 | Y = 1744.6X + 806.1 | 0.99 | 0.91 | 2.78 |
| 6 | Tannic acid | 1–100 | Y = 1816.4X − 953.8 | 0.99 | 0.21 | 0.63 |