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. 2016 Dec 12;55(1):516–525. doi: 10.1080/13880209.2016.1255976

Figure 1.

Figure 1.

HPLC profile of phenolic acids (λ = 280 nm) from cactus (Opuntia ficus-indica) cladodes (CCE). Six known phenolic acids identified in CCE: gallic acid (1), catechin (4), caffeic acid (5), epicatechin (6), vanillic acid (7) and coumarin (8). The HPLC separation of the active compounds was carried out on C-18 reverse phase HPLC column (Zorbax, 250 mm ×4.6 mm, particle size 5 μm) on an elution gradient at 25 °C. The mobile phase consisted of water:acetic acid (98:2 v/v) (A) and water:acetonitrile:acetic acid (58:40:2 v/v/v) (B). The elution gradient used was: 0–80% B for 25 min, 80–100% B for 10 min and 100–0% B for 5 min. The flow rate was 0.9 mL/min and the injection volume was 20 μL.