Table 2.
HPLC-DAD-ESI-MS characterization of phenolics in methanol extracts (ME) from leaves (L) and stems (S) of Helicteres vegae (Hv) and Heliopsis sinaloensis (Hs)a.
| Peak | tR (min) | UV λmáx (nm) | [M – H]−m/z | ESI-MSn m/z (% relative abundance) | Tentative identification | Extract of plant/tissue |
|---|---|---|---|---|---|---|
| F1 | 32.05 | 316, 266, 242 | 593 | MS2 [M – H]-: 593→447 (12.2), 307 (5), 285 (100) MS3 [M – H]-: 593→285→267 (30), 257 (100), 255 (4), 241 (50), 229 (45), 213 (43), 169 (20), 151 (83), 107 (7.5) |
Kaempferol 7-O- coumaroylhexoside | ME-LHv, ME-SHv |
| F2 | 27.94 | 346, 265, 240 | 593 | MS2 [M – H]-: 593→447 (2), 327 (6), 285 (100), 257(6) MS3 [M – H]-: 593→285→285 (98), 267 (50), 257 (100), 255 (3), 229 (43), 213 (28), 197 (18), 195 (10), 169 (5), 163 (18), 151 (15) |
Kaempferol 7-O-rhamnosylhexoside | ME-LHs |
| F3 | 28.47 | 345, 265, 240 | 593 | MS2 [M-H]-: 593→447 (2), 285 (100), 283 (3) MS3 [M-H]-: 593→285→257 (100), 255 (4), 229 (41), 213 (22), 195 (7), 162 (15.8), 151 (12) |
Kaempferol 7-O-rhamnosylhexoside | ME-LHs |
| P1 | 28.93 | 328, 247, 232 | 359 | MS2 [M-H]-: 359→ 223 (20), 197 (30), 179 (30), 161 (100) MS3 [M-H]-: 359→197 (100), 135 (18) |
Rosmarinic acid | ME-LHv, ME-SHv |
| P2 | 29.94 | 322, 247, 234 | 537 | MS2 [M – H]-:537 → 493 (100), 417 (13), 399 (60), 357 (5), 298 (10) MS3 [M – H]-: 537 →493 →359 (100), 313 (15), 295 (38), 179 (5) MS4 [M – H]-:537 →493 →359→ 223 (20), 197 (30), 179 (18), 161 (100), 135 (5) |
3′-O-(8″-Z-caffeoyl)-rosmarinic acid | ME-SHv |
| P3 | 33.9 | 295, 241 | Unknown | ME-LHs | ||
| P4 | 35.68 | 329, 295, 247 | Unknown | ME-LHs ME-SHs |
||
| P5 | 36.2 | 335, 266, 243 | Unknown | |||
| P6 | 36.72 | 323,289, 245 | Unknown | ME-LHs ME-SHs |
a
Under the evaluated conditions, MSn fragmentation of P3, P4, P5 and P6 was not possible.