Table 4.
Mutagenicity index (MI) and antimutagenicity (% of inhibition) of the methanol extracts (MEs) from Leaves (L) and Stems (S) of Helicteres vegae (Hv) and Heliopsis sinaloensis (Hs). The mutagenic agent 1-nitropyrene (1-NP) and the tester strain Salmonella enterica serovar Typhimurium YG1024 were used in the assay.
| Methanol extract | Concentration [μg/plate] | MIa | Revertants/platea | % of inhibitiona,b |
|---|---|---|---|---|
| Helicteres vegae | ||||
| Leaves (ME-LHv) | 1000 | 1.08 ± 0.2 | 135 ± 25 | 85.7 ± 4.67ξ |
| 500 | 1.21 ± 0.17 | 203 ± 43 | 78.5 ± 7.6#,& | |
| Stems (ME-SHv) | 1000 | 1.27 ± 0.22 | 236 ± 52 | 74.9 ± 9.10# |
| 500 | 0.99 ± 0.06 | 351 ± 9 | 63.3 ± 4.46* | |
| Heliopsis sinaloensis | ||||
| Leaves (ME-LHs) | 1000 | 1.14 ± 0.16 | 158 ± 3 | 83.4 ± 2.76&,ξ |
| 500 | 0.16 ± 0.3 | 243 ± 22 | 74.4 ± 6.03# | |
| Stems (ME-SHs) | 1000 | 1.28 ± 0.46 | 170 ± 4 | 82.1 ± 3.05&,ξ |
| 500 | 1.26 ± 0.23 | 227 ± 15 | 76.4 ± 1.94# | |
| 1-NP [100 ng/plate] | 965 ± 142 |
a
Results are the mean ± standard deviation of two independent experiments per triplicate.
b
Inhibition of the 1-NP (100 ng/tube) mutagenicity by the corresponding ME. Different symbols show significant differences (LSD = 5.67 p < 0.0001). The number of colonies due to spontaneous reversion was 69 ± 16.