Figure 2.

Identification of meisoindigo that inhibits zebrafish leukocyte recruitment to the injury site. (a) Chemical screening strategy. (b) Chemical structure of meisoindigo. (c,d) Tg:zlyz-EGFP embryos (3 dpf) without tail transection were treated with vehicle(n = 9) or meisoindigo(M, 100 μM, n = 11) for 6 h. Leukocytes around the whole trunk of zebrafish embryo [white boxes in (c)] were quantitiatively analyzed. (e,f) Tg:zlyz-EGFP embryos (3 dpf) with tail transection were treated with vehicle (n = 13), 25 μM (n = 13), 50 μM (n = 18) or 75 μM (n = 14) meisoindigo, respectively. Leukocytes that migrated to the highly inflamed regions [white boxes in (e)] at 6 hptt were quantitatively analyzed (f). The p values were annotated as obtained from a two-tailed t test.