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Meisoindigo has no effect on zebrafish leukocyte reverse migration from the injury site. (a) Treatment schedule. Tg:zlyz-EGFP embryos (3dpf) were subjected with tail transection and administrated with vehicle (n = 14) or meisoindigo (M, 100 μM, n = 11) from 6 hptt to 12 hptt. (b, c) Leukocytes migrated to the highly inflamed regions [white boxes in (b)] at 12hptt were quantitatively analyzed (c). The p value was annotated as obtained from a two-tailed t test.
