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. 2017 Apr 7;55(1):1545–1552. doi: 10.1080/13880209.2017.1309554

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Influence of BLIN on DHAV-1 adsorption, replication and release. Two sample-adding modes, pre-adding drug (A) and post-adding drug (B) were used to observe the influence of BLIN on DHAV-1 adsorption. The assay in each mode was repeated five times. (C) To observe the influence of BLIN on DHAV-1 replication, DHAV-1 was added to DEHs for 2 h to allow adsorption and penetration of the virus, and then BLIN at 20 μg/mL was added to DEHs for 10 h, five repetitions per treatment. (D) To observe the influence of BLIN on DHAV-1 release, DHAV-1 was added to DEHs for 32 h to allow adsorption, penetration, replication and release, and then BLIN at 20 μg/mL was added to DEHs for 1 h, five repetitions per treatment. The qRT-PCR method was used to measure the relative DHAV-1 gene expression. Statistical analyses were performed by t tests. ND: not detected; *Compared with virus control, p < 0.05; **Compared with virus control, p < 0.01.