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. 2017 Jun 23;55(1):1899–1908. doi: 10.1080/13880209.2017.1311351

Figure 6.

Figure 6.

Analysis of the activation of caspases-3/7, -8 and -9 during 4-NRC-induced apoptosis. The cells (1 × 106 cells/mL) were treated with 4-NRC (27 μM) for 24 h and analyzed using a flow cytometer. To evaluate the involvement of ROS in caspase activation, cell groups were also pretreated with N-acetyl-l-cysteine (NAC) (2 mM) for 1 h following treatment with 4-NRC. Each bar presents mean ± SD of three independent experiments (**p < 0.01 and ***p < 0.001 vs. control).