Table 2.
Chromosomal aberrations induced by DMBA and DMBA plus Turkish black tea (TBT) extract in vivo on bone marrow cells of mice.
| Treatment (mg/kg) | Metaphase cells scored | Gapsa | Aberrations/cellsb |
Aberrant cells % (Mean ± S.D.)c | Mitotic indices (Mean ± S.D.)c | |
|---|---|---|---|---|---|---|
| Chromatid type | Chromosome type | |||||
| DMBA (50mg/kg) | 300 | 31 | 0.127 | 0.073 | 17.00 ± 2.28 | 1.57 ± 0.24 |
| TBT 300 + DMBA | 300 | 28 | 0.117 | 0.037 | 14.67 ± 3.08 | 1.72 ± 0.29 |
| TBT 600 + DMBA | 300 | 25 | 0.127 | 0.047 | 15.67 ± 2.52 | 1.66 ± 0.16 |
| TBT 1200 + DMBA | 300 | 23 | 0.103 | 0.030 | 12.33 ± 1.27* | 1.62 ± 0.25 |
| Water control | 300 | 11 | 0.133 | 0.040 | 4.35 ± 1.68 | 2.18 ± 0.27 |
| Only TBT 1200 | 300 | 16 | 0.147 | 0.093 | 6.00 ± 1.68 | 1.93 ± 0.29 |
a
Total chromatid and chromosome gaps at each dose were recorded but not included as aberrations/cell.
b
Total number of aberrations (chromatid and chromosome type)/total number of cells scored per dose. Results are of 4 animals (75 metaphase cells/animal).
c
Results at each dose were compared to those of control using Dunnett’s multiple comparison with control.
*
p < 0.05 when compared with DMBA treated only. TBT 300 + DMBA:300 mg/kg TBT + DMBA; TBT 600 + DMBA:600 mg/kg TBT + DMBA; TBT 1200 + DMBA:1200 mg/kg TBT + DMBA; Only TBT 1200:1200 mg/kg TBT. DMBA: dimethylbenz(a)anthracene.