Table 3.

Histopathological changes in liver of rats treated with β-aescin.

Groups	Observations	Score
Normal control	Normal architecture of liver	0
CCl4 control	Large numbers of inflammatory cells such as lymphocytes along with hepatic sinusoidal inflammation, vacuolization of cytoplasm, fatty vacuoles and hepatocyte necrosis	+++
Silymarin 50 mg/kg treated	Area of regeneration and dark nucleus	#
β-Aescin 0.9 mg/kg treated	Presence of less clusters of inflammatory cells and absence of hepatocyte necrosis	++
β-Aescin 1.8 mg/kg treated	Very few inflammatory lesions along with prominent nucleolus	+
β-Aescin 3.6 mg/kg treated	Marked focal regenerative changes	#

0: indicates no score is evaluated.

+++ (severe) indicates when ≥50% area of liver is affected by inflammatory cells and necrotic lesions.

++ (moderate) indicates when 25–50% area of liver is affected by inflammatory cells and necrotic lesions.

+ (mild) indicates when ≤25% area of liver is affected by inflammatory cells and necrotic lesions.

#indicates when regenerating area and prominent nucleolus has been noticed.