Figure 3.

The high performance of liquid chromatograms (HPLC) of condensed tannin containing fraction’s separated fractions. (A) Phenolic acids containing monomeric fraction. (B) Catechin, oligomeric condensed tannin containing fraction. (C) Polymeric condensed tannin containing fraction. The mobile phase consisted of 2.5% (v/v) aqueous acetic acid solution (solvent A) and acetonitrile (solvent B). The gradient program consisted of 3% B, initially, changing to 21% B after 4 min, was maintained at 21% B until 10 min and raised to 22% B after 11 min, was maintained at 22% B until 15 min and raised to 35% B after 16 min, was maintained at 35% B until 35 min and raised to 100% B after 36 min, and was maintained at 100% B until 40 min and then reduced to 3% B after 45 min. The injection volume of all the samples was 20 μL. Simultaneous monitoring was performed at 280 nm, and the flow rate was 0.8 mL/min.