Figure 6.

The effect of beetroot on the cells committed to hematopoiesis of bone marrow in irradiated mice. Mice administered with beetroot extract were exposed to 7 Gy irradiation and bone marrow cells of femurs were isolated from mice of each experiment group 10 days after irradiation. (a) Cell cycle of bone marrow cells of irradiated mice. Fluorescence-activated cell sorting determined the portion of bone marrow cells in the sub-G1 phase (apoptotic peak) of the cell cycle. (b–d) Representative images of Ki-67 immunoperoxidase staining in the femurs of (b) Naive, (c) IR and (d) IR + Beetroot groups. Bars =50 μm. (e–g) Representative images of immunoperoxidase staining of c-kit in the femurs of (e) non-irradiated, (f) irradiated and (g) IR + Beetroot groups. Bars =50 μm. (H-I) Quantification of (h) Ki-67 and (I) c-kit positive cells performed using five lesions showing the most representative expression of each mouse (3 mice per group). Data are represented as means ± SEM of three independent experiments (*p < .05, **,††p < .01, ***,†††p < .001).