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. Author manuscript; available in PMC: 2019 Feb 13.
Published in final edited form as: Nat Struct Mol Biol. 2018 Aug 13;25(9):778–786. doi: 10.1038/s41594-018-0106-9

Figure 1. A forward genetic screen identifies novel antiviral immunity genes.

Figure 1

a, Diagram of the lys-3p::gfp viral stress sensor.

b, Ovid screen workflow. Transgenic animals carrying the viral stress sensor were mutagenized using EMS and F2 progeny were assayed. OrV, Orsay virus. Ovid, Orsay virus immunodeficient.

c, Top panel: viral load of strains as indicated, measured by qRT-PCR of OrV RNA1, 4 dpi. Bars represent average value; error bars represent the standard error of the mean (SEM) of four independent infections. One-tailed student’s t-test: ****p<0.0001, *** p<0.001, **p<0.01, *p<0.05. Bottom panel: locomotion defects scored (paralyzed or twitching) after unc-22 RNAi feeding. Bars: average value; error: SEM; three independent RNAi treatments. Two-tailed student’s t-test: ****p<0.0001, **p<0.01.

d, Viral load compared to sdz-6 mRNA levels by qRT-PCR. Dots: average value; error: SEM; four independent infections. Samples as in c.