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. Author manuscript; available in PMC: 2019 Feb 13.
Published in final edited form as: Nat Struct Mol Biol. 2018 Aug 13;25(9):778–786. doi: 10.1038/s41594-018-0106-9

Figure 3. CDE-1 acts in parallel to antiviral RNAi.

Figure 3

a, Schematic of antiviral RNAi in C. elegans. Viral Recognition Complex (ViRC) includes DCR-1; DRH-1; RDE-4.

b, Comparison between the viral load and primary viRNA populations. Primary viRNAs (23-nucleotide long, from 5′ monophosphate RNA sequencing). Only antisense RNAs were considered to exclude potential viral genome degradation products. Dots: independent infection.

c, Comparison between the viral load and secondary viRNA populations. Secondary viRNAs (22-nucleotide long, starting with a G, from 5′ tri/monophosphate RNA sequencing). Samples as in b.

d and e, Viral load as measured by qRT-PCR of OrV RNA1 genome in adults two days after infection. Boxplots: whiskers from minimum to maximum; dots: independent infection; n=5. One-tailed student’s t-test: *** p<0.001, **p<0.01, *p<0.05. Samples as in b.