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. 2018 Sep 10;13(9):e0203118. doi: 10.1371/journal.pone.0203118

Fig 1. Increased autoantibody production in NZB mice neonatally infected with LCMV.

Fig 1

Mice were infected <24 h after birth with LCMV Arm or Cl13 and analyzed at the indicated ages. Uninfected mice were used as controls. (A) Total serum IgG titers detected by ELISA (n = 9–18 mice). (B) Incidence and levels of red blood cell (RBC)-bound autoantibodies determined by FACS (n = 7–18 mice). (C) Anti-nuclear autoantibodies assessed by incubating mouse serum with HEp-2 cells and immunofluorescence analysis (n = 9–18 mice). (D) Anti-chromatin IgG2a autoantibodies detected by ELISA (sensitivity 5–10 ng/mL, n = 5–9 mice). Graphs summarize data from 2–3 independent experiments. Dots (in A-C) represent individual mice, horizontal bars (in A-C) indicate average, error bars (in D) indicate standard deviation, and asterisks indicate statistical significance (*, p<0.05; **, p<0.01; ***, p<0.001).