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. 2018 Aug 28;14(8):e1007278. doi: 10.1371/journal.ppat.1007278

Fig 1. Specific affinity of α4β7 for HIV gp120, MAdCAM and SIV gp120.

Fig 1

A) Sensorgram of increasing concentrations (2-fold) of soluble α4β7 passed over surface-immobilized MAdCAM in the presence of 1 mM MnCl2 for 120 sec, followed by a 120 sec washout/dissociation phase. Mass of bound α4β7 (y-axis) expressed as response units (RU). Affinity expressed as KD (nM) is shown. B) Same as in panel A, with immobilized A244 gp120. C) Same as in panel A with immobilized MAdCAM in the absence of divalent cations. D) Same as in panel A with immobilized A244 gp120 in the absence of divalent cations. E) Same as in panel A with immobilized cV2 92TH023 peptide. F) Same as in panel A with immobilized 92TH023 cV2 peptide and soluble α4β1 as the analyte. G) Same as in panel A with immobilized cV2 C06980v0c22 peptide. H) Same as in panel A with immobilized BG505 gp120. I) Same as in panel A with immobilized SIVmac766 gp120. Additional binding parameters are listed in S1 Table. Each sensorgram is representative of three independent measurements of each analyte-ligand interaction.