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. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 31;1006:167–178. doi: 10.1016/j.jchromb.2015.10.030

Figure 4.

Figure 4

Extracted ion chromatograms of a standard mixture of dATP, dCTP, dGTP, dTTP, ATP, CTP, GTP, and UTP (200 nM in aqueous solution), using the TEA-HFIP ion-paring mobile phase with different gradient slope of organic solvent. Chromatographic separation was performed on an Atlantis T3 (2.1× 100 mm, 3 μm) column, under a gradient mobile phase consisting of mobile phase A (8.6 mM TEA + 100 mM HELP) and mobile phase B containing 100% (A), 50% (B), or 10% (C) acetonitrile in mobile phase A. The gradient program was set as B% (min): 0(0) → 10 (6.0) → 0 (6.1) → 0 (10).