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. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 31;1006:167–178. doi: 10.1016/j.jchromb.2015.10.030

Figure 5.

Figure 5

Multiple-reaction monitoring (MRM) chromatograms of dATP (490.0 > 392.1), dCTP (466.0 > 367.9), dGTP (506.0 >256.9), dTTP (480.9 > 383.1), ATP (506.0 > 272.9), CTP (482.0 > 384.1), GTP (522.0 > 424.0), and UTP (483.0 > 384.9) for: (A1-A8) a standard mixture (containing 200 nM of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP) in aqueous solution; (B1-B8) a cellular extract sample diluted 20-fold with the mobile phase A (8.6 mM TEA + 100 mM HFIP); and (C) the undiluted cellular extract sample (containing ~10 million cells). Chromatographic separation was achieved on an Atlantis T3 (2.1× 100 mm, 3 μm) column, under gradient mobile phase consisting of mobile phase A (8.6 mM TEA + 100 mM HFIP) and mobile phase B (10% acetonitrile in mobile phase A), with gradient B% (min) as 0 (0) → 10 (6.0) → 0 (6.1) → 0 (10) at a flow rate of 0.5 mL/min.