. Author manuscript; available in PMC: 2018 Sep 10.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 31;1006:167–178. doi: 10.1016/j.jchromb.2015.10.030

Table 1.

Optimized mass spectrometric parameters and chromatographic retention time for individual NTPs, dNTPs, and their respective isotope-labeled internal standard

Analyte	MS Transition (m/z)	Collision energy (V)	Decluster Potential (V)	Retention Time (min)
ATP	506.0 > 272.9	−40	−100	3.78
CTP	482.0 > 384.1	−31	−100	2.77
GTP	522.0 > 424.2	−30	−100	2.98
UTP	483.0 > 385.0	−27	−100	2.74
dATP	490.0 > 392.1	−31	−100	4.96
dCTP	466.0 > 368.0	−30	−100	3.27
dGTP	506.0 > 257.0	−44	−100	3.91
dTTP	480.9 > 383.0	−29	−100	3.97
ATP-¹³C₁₀¹⁵N₅	521.1 > 278.1	−45	−100	3.76
CTP-¹³C₉¹⁵N₃	494.1 > 396.0	−28	−100	2.77
GTP-¹³C₁₀¹⁵N₅	537.0 > 439.0	−30	−100	2.98
UTP-¹³C₉	492.0 > 394.1	−29	−100	2.75
dATP-¹³C₁₀¹⁵N₅	505.1 > 407.0	−33	−100	4.94
dCTP-¹³C₉¹⁵N₃	478.1 > 380.1	−31	−100	3.25
dGTP-¹³C₁₀¹⁵N₅	521.0 > 262.0	−45	−100	3.89
dTTP-¹³C₁₀¹⁵N₂	493.0 > 395.0	−29	−100	3.95