Table 2.
Intra- and inter-day precisions and accuracy for quality control (QC) samplesa.
| Compound | Nominal Conc. (nM) | Determined Conc. (nM)c | Accuracy (%)d | Intra-day (%) | Inter-day (%) |
|---|---|---|---|---|---|
| ATP | 1 (LLOQ) | 1.1±0.2 | 114.0 | 18.7 | 9.7 |
| 3 | 2.8±0.2 | 93.3 | 6.7 | 4.6 | |
| 200 | 194.6±14.0 | 97.3 | 6.5 | 3.8 | |
| 400 | 406.6±32.7 | 101.6 | 6.7 | 5.8 | |
| CTP | 0.5 (LLOQ) | 0.52 ± 0.06 | 103.5 | 12.2 | 2.3 |
| 1.5 | 1.5 ± 0.2 | 102.3 | 12.2 | -b | |
| 200 | 194.1 ± 14.2 | 97.0 | 7.4 | -b | |
| 400 | 396.5 ± 18.8 | 99.1 | 3.6 | 4.1 | |
| GTP | 5 (LLOQ) | 4.9 ± 0.8 | 97.8 | 15.7 | 4.9 |
| 15 | 16.2 ±1.9 | 108.1 | 12.3 | -b | |
| 200 | 197.7 ±16.2 | 98.8 | 8.5 | -b | |
| 400 | 413.6 ± 27.5 | 103.4 | 4.6 | -b | |
| UTP | 0.5 (LLOQ) | 0.58 ±0.08 | 115.2 | 14.9 | -b |
| 1.5 | 1.5 ± 0.1 | 99.4 | 7.7 | 3.9 | |
| 200 | 201.2 ±15.3 | 100.6 | 8.0 | -b | |
| 400 | 399.3 ± 30.6 | 99.8 | 7.4 | 2.5 | |
| dATP | 0.5 (LLOQ) | 0.53±0.07 | 105.4 | 16.4 | 7.2 |
| 1.5 | 1.4±0.1 | 95.2 | 10.1 | 2.0 | |
| 200 | 200.4±17.6 | 100.2 | 4.0 | 9.7 | |
| 400 | 410.2±38.7 | 102.6 | 5.8 | 9.7 | |
| dCTP | 0.5 (LLOQ) | 0.52±0.05 | 104.2 | 9.8 | -b |
| 1.5 | 1.4±0.1 | 92.5 | 7.8 | 5.1 | |
| 200 | 189.2±10.8 | 94.6 | 5.9 | -b | |
| 400 | 373.0±18.9 | 93.2 | 4.4 | 3.0 | |
| dGTP | 5 (LLOQ) | 5.6±0.7 | 111.6 | 10.3 | 9.8 |
| 15 | 1 3. 1 ±1 .5 | 87.3 | 10.6 | 4.7 | |
| 200 | 201.3±15.6 | 100.6 | 5.4 | 7.2 | |
| 400 | 442.9±20.9 | 110.7 | 4.6 | 1.4 | |
| dTTP | 0.5 (LLOQ) | 0.54±0.07 | 108.1 | 7.8 | 13.4 |
| 1.5 | 1.4±0.1 | 93.8 | 8.3 | 3.3 | |
| 200 | 199.9±11.5 | 100.0 | 3.0 | 6.4 | |
| 400 | 392.4±14.2 | 98.1 | 2.2 | 3.8 |
a
Each QC was performed in quintuplicate on three different days.
b
No additional variation was observed as a result of performing assay in different days.
c
Data presented as mean ± standard deviation of 15 replicates (quintuplicate on three different days).
d
Calculated as mean determined concentration / nominal concentration (n = 15).