Table 3. Fractions of A, T, G, and C in Each Primer Set, from Each Supplier, Determined by Deep Sequencing.
| primer set | supplier | A | T | G | C |
|---|---|---|---|---|---|
| NNNa | 1 | 9% (23 183/258 114) | 41% (106 320/258 114) | 34% (87 976/258 114) | 16% (40 665/258 114) |
| 2 | 16% (40 428/251 781) | 38% (95 853/251 781) | 31% (76 807/251 781) | 15% (38 691/251 781) | |
| 3 | 24% (56 992/240 975) | 26% (61 972/240 975) | 28% (68 464/240 975) | 22% (53 547/240 975) | |
| NNBb | 1 | 13% (33 350/256 731) | 39% (98 622/256 731) | 28% (72 912/256 731) | 20% (51 847/256 731) |
| 2 | 13% (32 967/266 253) | 35% (93 144/266 253) | 34% (89 211/266 253) | 18% (47 073/266 253) | |
| 3 | 15% (39 392/259 257) | 29% (74 528/259 257) | 31% (81 671/259 257) | 25% (63 666/259 257) |
a
In NNN, the expected fractions of A, T, G, and C were 25%.
b
In NNB, the expected fractions were 16% A and 28% each of T, G, and C.