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. 2018 Jul 13;293(36):14001–14011. doi: 10.1074/jbc.RA118.003099

Figure 1.

Figure 1.

Nur77 increases relaxin-3 expression in neonatal rat cardiomyocytes. A, expression of RLN1, RLN3, and RXFPs in NRVMs, as determined by RT-PCR; B, effects of orphan nuclear receptor Nur77 and NOR-1 on the expression of relaxin-3 in NRVMs. NRVMs were transduced with the indicated adenoviruses at an MOI of 50. 48 h after transduction, the expression of RLN3 was then determined by real-time PCR (n = 4); *, p < 0.05 compared with Ad-LacZ. B, amplicons of RLN1, RLN3, and Nur77 were detected by real-time PCR. C, Nur77 increases RLN3, but not RLN1, expression in a dose-dependent manner. NRVMs were transduced with the indicated MOIs of adenoviruses. 48 h after transduction, expression of RLN1 and RLN3 was determined by real-time PCR (n = 4); *, p < 0.05 compared with Ad-LacZ at MOI = 50. Expression of Nur77 and GAPDH was determined by Western blotting. D, increased levels of RLN3 in the supernatants of NRVMs transduced with Ad-Nur77. NRVMs were transduced with the indicated adenoviruses at MOI of 30. 48 h after transduction, the levels of RLN3 in the supernatants were determined by ELISA (n = 4). *, p < 0.05 compared with Ad-LacZ at MOI = 50. E, effects of Nur77, NOR1, and DN-Nur77 on the RLN3 expression in cardiomyocytes. NRVMs were transduced with the indicated adenoviruses at MOI = 30. 48 h after transduction, expression of RLN3 was determined by qRT-PCR (n = 4). *, p < 0.05 compared with Ad-LacZ. Error bars, S.D.