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. 2018 Jul 13;293(36):14001–14011. doi: 10.1074/jbc.RA118.003099

Figure 3.

Figure 3.

ISO up-regulates expression of Nur77 and RLN3 in cardiomyocytes. A, NRVMs cultured in 6-well plates were starved in serum-free medium for 48 h and then stimulated with ISO (10 μm) for the indicated times. The expression of Nur77 and RLN3 was determined by real-time PCR (n = 4). *, p < 0.05 compared with time at 0 h; #, p < 0.05 compared with time at 0 h. B, NRVMs cultured in 6-well plates were starved in serum-free medium for 48 h and then stimulated with indicated concentrations of ISO. The expression of Nur77 and RLN3 was determined at 1 and 6 h, respectively, after ISO stimulation by real-time PCR (n = 4). *, p < 0.05 compared with ISO at 0 μm; #, p < 0.05 compared with ISO at 0 μm. C, NRVMs cultured in 6-well plates were starved in serum-free medium for 48 h and then pretreated with the indicated inhibitors for 1 h and then stimulated with either 10 μm ISO or sterile 0.9% saline for 6 h. The expression of RLN3 was determined by real-time PCR; *, p < 0.05 compared with saline; #, p < 0.05 compared with ISO alone at 10 μm. D, NRVMs cultured in 6-well plates were starved in serum-free medium for 48 h and then stimulated with either 0.9% saline solution, 10 μm ISO, 10 μm PE, or 100 nm ET-1 for 6 h. The expression of RLN3 was then determined by real-time PCR (n = 4). *, p < 0.05 compared with saline. Error bars, S.D.