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. 2018 Jul 24;293(36):13974–13988. doi: 10.1074/jbc.RA118.003541

Figure 2.

Figure 2.

Adipose tissue inflammation is enhanced in Plin1−/− mice. A, relative mRNA levels of inflammatory cytokine genes (Mcp-1 and Tnfα) and macrophage markers (F4/80 and Cd11c) were measured in eWAT by qRT-PCR. B, serum levels of MCP-1 and TNFα were assessed by ELISA. C, macrophage accumulation was detected in eWAT from Plin1+/+ and Plin1−/− mice by immunohistochemistry analysis of the nuclei (blue), CD11b (red), and CD11c (green). Scale bars, 50 μm. D–I, macrophage accumulation was measured in eWAT by flow cytometric analysis. The percentages of F4/80+CD11b+ (D) and F4/80+CD11b+CD11c+ (E) cells in the SVCs of eWAT are shown in the graphs. Total numbers of F4/80+CD11b+ (F) and F4/80+CD11b+CD11c+ (G) cells in SVCs/g of eWAT were determined. Percentages of CD11c+ (H) and CD206+ (I) cells in the F4/80+CD11b+ cells were measured. All data represent the mean ± S.E. *, p < 0.05; **, p < 0.01 versus Plin1+/+ group by Student's t test. All qRT-PCR data were normalized to the mRNA level of cyclophilin.