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. 2018 Jul 18;293(36):14134–14145. doi: 10.1074/jbc.RA118.002426

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© 2018 Dijk et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — Cleavage of LPL in human and mouse adipose tissue. A, Western blotting of lysates of human adipose tissue using antibodies against the N-terminal (Y-20) and C-terminal (88b8) portion of hLPL. B, Western blotting of lysates of human adipose tissue treated with EndoH or PNGase, using two different antibodies against hLPL, 88b8, and 5D2 (27). C, Western blotting of lysates of human adipose tissue of different subjects enrolled in the MONDIAL study, using an antibody against hLPL (88b8). D, Western blotting of lysates of mouse adipose tissue treated with EndoH or PNGase, using an antibody against mLPL (28). E, Western blotting of lysates of mouse heart and bone marrow–derived macrophages. Molecular weight markers are indicated. l.e., long exposure; s.e., short exposure.