Figure 10.

Down-regulation of Angptl4 by insulin reduces LPL cleavage. A, expression of Angptl4 and Lpl in primary mouse adipocytes treated with insulin (500 nm) for 12 h. **, significantly different from control according to Student's t test; p < 0.01. B, Western blotting of cell lysates of primary adipocytes differentiated from the stromal vascular fraction of adipose tissue from WT and Angptl4^−/− mice treated with 500 nm insulin for 12 h. C, Western blotting of cell lysates of primary adipocytes differentiated from the stromal vascular fraction of adipose tissue from WT and Angptl4^−/− mice treated with 500 nm insulin for 12 h. Adipocyte lysates were treated with EndoH. Western blots were probed with antibodies against mLPL. Coomassie Blue staining was performed as loading control. R, EndoH-resistant LPL (complex oligosaccharides: Golgi and cell surface LPL). S, EndoH-sensitive LPL (high-mannose oligosaccharides, ER LPL). FL, full-length; N-ter, N-terminal. Error bars, S.D.