Table 1.

¹H/¹³C NMR chemical shift assignments of compound 3 (δ, ppm; J, Hz)

The data (δ) were measured in HOD (D 99.9%). The assignments were based on HSQC, COSY, TOCSY, ROESY, and HMBC experiments.

	Octasaccharide (3)
	dF	dU	dA	Fuc	GlcA	rA	rF	rU
H1	5.20 J (1,2) = 4.32	4.85 J (1,2) = 8.64	4.53 J (1,2) = 8.56	5.28 J (1,2) = 4.16	4.43 J (1,2) = 7.60	4.63 J (1,2) = 7.60	5.04 J (1,2) = 4.08	3.73 J (1,1′) = 11.76
								3.69 (H-1′) J (1/1′,2) = 3.92, 6,72
C1	100.91	105.74	102.38	101.99	106.34	104.17	104.04	65.33
H2	3.88 J (2,3) = 10.40	3.83 J (2,3) = 8.32	4.08 J (2,3) = 9.52	3.87 J (2,3) = 10.48	3.55 J (2,3) = 8.36	3.98	3.89 J (2,3) = 10.56	4.07 J (2,3) = 3.92
C2	69.18	72.99	54.27	69.12	76.20	54.27	69.34	72.56
H3	4.53 J (3,4) = 2.96	4.42 J (3,4) = 2.72	4.10 J (3,4) = 2.52	4.46 J (3,4) = 2.88	3.63 J (3,4) = 8.72	3.97 J (3,4) = 0.80	4.55 J (3,4) = 3.36	4.02 J (3,4) = 3.92
C3	77.97	79.09	78.88	78.11	82.00	78.47	77.97	82.10
H4	4.83	5.68	4.91	4.96	3.95 J (4,5) = 9.32	4.75	4.83	4.01 J (4,5) = 4.08
C4	81.70	109.42	78.98	82.10	77.95	78.98	81.66	84.20
H5	4.27 J (5,6) = 6.72		4.01 J (5,6/6′) = 5.28, 8.56	4.88 J (5,6) = 6.48	3.64	4.01 J (5,6/6′) = 4.56, 8.72	4.36 J (5,6) = 6.56	4.25
C5	69.24	149.61	74.74	69.00	79.91	75.04	69.55	74.64
H6	1.23		4.32 J (6,6′) = 11.96	1.34		4.22 J (6,6′) = 10.96	1.24
			4.22 (H-6′)			4.12 (H-6′)
C6	18.56	171.62	69.99	18.79	177.73	70.50	18.83	180.09
H7
C7			177.72			177.72
H8			1.99			2.00
C8			25.26			25.29