Fig. 1.

Phloxine O inhibits TSLP production in keratinocytes. (A) Chemical structure of phloxine O. (B) PAM212 cells were treated with phorbol 12-myristate 13-acetate (PMA, 30 nM) or vehicle (0.2% ethanol) in the presence or absence of Phloxine O (PO) for 16 hours. (C) KCMH-1 cells were treated with Phloxine O (PO) or vehicle (0.2% ethanol) for 16 hours. TSLP protein levels in culture media were determined by ELISA. For (B) and (C), data are shown as the mean ± SEM (n=3). ^#Significantly different from vehicle alone, p<0.05. ^*Significantly different from PMA alone, p<0.05. (D) PAM212 cells were treated with Phloxine O for 24 hours, and the cytotoxicity was determined by LDH release assay. The LDH release of vehicle group was set up as 100%. Data are shown as the mean ± SEM (n=6).