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. 2018 Sep 7;5(6):e500. doi: 10.1212/NXI.0000000000000500

Figure 3. Impaired antiviral and inflammatory responses to the POL III ligand poly(dA:dT) and increased VZV replication in patient PBMCs.

Figure 3

PBMCs from P1 and 9 healthy controls were used for experiments. (A–C) PBMCs were transfected with poly(dA:dT) (2 μg/mL) for 6 hours. (D–F) PBMCs were transfected with poly(I:C) (2 μg/mL) for 6 hours. (G–K) VZV-infected MeWo cells were cocultured with PBMCs for 48 hours. Total RNA was isolated for measurement of mRNA for the indicated cytokines by reverse transcription-qPCR. (L), VZV replication was assessed by measuring the viral transcript ORF63. Cytokine levels and ORF63 levels were normalized to TBP and compared with pooled results of 9 healthy controls. Data are shown as column bars, and error bars represent SD. The nonparametric Mann-Whitney rank-sum test was used to evaluate statistical significance between groups. Significance level *p < 0.05 and **p < 0.001. mRNA = messenger RNA; PBMC = peripheral blood mononuclear cell.